Skip to content
BACwater.ai
Mitochondrial and aging research

Epithalon reconstitution calculator and reference

Research use: limited or no human data

Also spelled Epitalon. Both spellings refer to the same peptide.

Category
Longevity
Common vial sizes
10, 20, 50 mg
Shelf life
30 days
refrigerated, mixed

Short answer: add 2 mL of bac water to a 10 mg Epithalon vial. A 5 mg amount then measures about 100 units on a 1 mL (U-100) insulin syringe.

To mix a 10 mg vial of Epithalon, add about 2 mL of BAC water. Now each 1 mL of liquid holds 5 mg of peptide. So a 5 mg dose is about 100 units on a 1 mL insulin syringe. Want a rounder number? Add a little more or a little less water.

What nobody knows

  • How much, if any, is safe for a person.
  • Whether it does anything in people, and what it does over the long term.
  • What is actually in your vial: the powder's identity, purity, and strength.
Compiled and maintained by the BACwater.ai editorial team and checked against the sources cited on this page. This is general research information, not a medical review. Last updated July 2026.

Epithalon bac water calculator

= 5 mg

Add this much bac water
2mL
Concentration
5 mg/mL
Per dose
100 units
Doses / vial
2

This is general reconstitution math for research and educational use only. BACwater.ai is not a medical company, and this is not medical advice. Always check your product's own paperwork and talk to your doctor before making any health decision. Read the full disclaimer.

What you cannot know about your vial

  • You cannot see what is really inside. Independent testing in this market has found research powders that were mislabeled, weaker or stronger than the label, or contaminated. An unlabeled vial tells you nothing you can verify.
  • “Research-grade” is not a standard. It is not a grade anyone checks. It does not promise that the powder is Epithalon, that it is pure, or that the amount matches the label.
  • No calculation fixes this. The math here is exact for the numbers you type. It cannot tell you whether the powder in your vial matches what is printed on it.
Read more: what you cannot know about your vial

What is Epithalon?

Epithalon is a small lab-made peptide (it is made of four building blocks, so it is called a tetrapeptide). It is studied in longevity and cellular research.

It is commonly researched in cycles. Reconstitution follows the standard method.

Longevity research peptides are reconstituted using the standard method. Once mixed, they follow the same refrigerated shelf-life rules as other peptides.

How much bac water for Epithalon?

The chart below is a Epithalon reconstitution chart: each common vial size, the bac water to add, and where a 5 mgmeasurement lands on a 1 mL insulin syringe. Use the calculator above for your exact vial and the amount you want to measure.

Vial amountBac water to addConcentrationAmount to measureSyringe units
10 mg2 mL5 mg/mL5 mg100 units
20 mg1 mL20 mg/mL5 mg25 units
50 mg1 mL50 mg/mL5 mg10 units

The amount in the “Amount to measure” column is an example chosen so the math is easy to follow. It is not a recommended amount, and this site does not recommend how much to use. Units assume a U-100 insulin syringe (100 units = 1 mL). Always confirm the amount printed on your own vial.

Epithalon: concentration by vial size after reconstitutionEpithalon reconstitution reference: a 10 mg vial with 2 mL of bac water makes 5 mg/mL; a 20 mg vial with 1 mL of bac water makes 20 mg/mL; a 50 mg vial with 1 mL of bac water makes 50 mg/mL. Concentration is the vial amount divided by the water added; no dose is assumed. BACwater .ai RECONSTITUTION REFERENCE Epithalon Concentration you get at each common vial size and bac water amount. 10 mg + 2 mL water 5 mg/mL 20 mg + 1 mL water 20 mg/mL 50 mg + 1 mL water 50 mg/mL Concentration = vial amount ÷ water added. Longer bar = stronger liquid. No dose is assumed and nothing is for sale.
Epithalon reconstitution reference: a 10 mg vial with 2 mL of bac water makes 5 mg/mL; a 20 mg vial with 1 mL of bac water makes 20 mg/mL; a 50 mg vial with 1 mL of bac water makes 50 mg/mL. Concentration is the vial amount divided by the water added; no dose is assumed.

How to reconstitute Epithalon

  1. 1
    Gather your supplies

    Wash your hands and lay out your Epithalon vial, a vial of bacteriostatic water, an insulin syringe, and alcohol prep pads on a clean surface.

  2. 2
    Swab both vial tops

    Wipe the rubber stopper of both the peptide vial and the bacteriostatic water vial with separate alcohol prep pads. Let them air dry.

  3. 3
    Draw the bacteriostatic water

    For a 10 mg vial, draw about 2 mL of bacteriostatic water. Use the calculator on this page to match your exact vial strength.

  4. 4
    Add water to the peptide vial

    Insert the needle at an angle and let the water run slowly down the inside wall of the vial. Do not spray it directly onto the powder.

  5. 5
    Swirl gently

    Roll or swirl the vial between your palms until the powder fully dissolves. Roll it, do not shake it, if your product's instructions say so. The solution should look clear.

  6. 6
    Label and refrigerate

    Label the vial with the peptide name, the date mixed, and the expiration date, then refrigerate it immediately.

Storage and shelf life

30 days
refrigerated

Keep in the fridge. Once you add bac water, the peptide slowly breaks down, so refrigerate the vial and discard it if the solution turns cloudy or develops particles.

Epithalon reconstitution FAQ

Add about 2 mL of bacteriostatic water to a 10 mg vial of Epithalon. That creates a 5 mg/mL solution, so a 5 mg dose is about 100 units on a 1 mL insulin syringe. Adjust the water amount to move the dose to a cleaner mark.

Add about 1 mL of bacteriostatic water to a 20 mg vial of Epithalon. That creates a 20 mg/mL solution, so a 5 mg dose is about 25 units on a 1 mL insulin syringe. Adjust the water amount to move the dose to a cleaner mark.

Add about 1 mL of bacteriostatic water to a 50 mg vial of Epithalon. That creates a 50 mg/mL solution, so a 5 mg dose is about 10 units on a 1 mL insulin syringe. Adjust the water amount to move the dose to a cleaner mark.

Once mixed, Epithalon is typically stable for about 30 days when refrigerated. Keep in the fridge. Discard it sooner if the solution turns cloudy or develops particles.
Advertisement

Related peptides

Compare Epithalon with MOTS-c side by side

References

Primary sources for the facts on this page. We cite regulatory and peer-reviewed authorities rather than secondary blogs.

  1. Bacteriostatic Water for Injection, USP - prescribing information · U.S. FDA labeling via DailyMed (NIH / NLM)Defines bacteriostatic water as sterile water with 0.9% benzyl alcohol added as a bacteriostatic preservative, supplied in a multiple-dose container for diluting or dissolving drugs; contraindicated in neonates.
  2. Benzyl alcohol (Compound CID 244) · NIH PubChem, National Library of MedicineChemical identity, properties, and safety data for benzyl alcohol, the bacteriostatic preservative in BAC water.
Ready to mix Epithalon?

The Plan Builder turns these numbers into a printable, step-by-step plan. You can also read how reconstitution works, brush up on the bac water calculator, or start from the complete bac water guide.