GHK-Cu reconstitution calculator and reference
Also called copper peptide.
Short answer: add 2.5 mL of bac water to a 50 mg GHK-Cu vial. A 2 mg amount then measures about 10 units on a 1 mL (U-100) insulin syringe.
To mix a 50 mg vial of GHK-Cu, add about 2.5 mL of BAC water. Now each 1 mL of liquid holds 20 mg of peptide. So a 2 mg dose is about 10 units on a 1 mL insulin syringe. Want a rounder number? Add a little more or a little less water.
What nobody knows
- How much, if any, is safe for a person.
- Whether it does anything in people, and what it does over the long term.
- What is actually in your vial: the powder's identity, purity, and strength.
GHK-Cu bac water calculator
= 2 mg
This is general reconstitution math for research and educational use only. BACwater.ai is not a medical company, and this is not medical advice. Always check your product's own paperwork and talk to your doctor before making any health decision. Read the full disclaimer.
What you cannot know about your vial
- You cannot see what is really inside. Independent testing in this market has found research powders that were mislabeled, weaker or stronger than the label, or contaminated. An unlabeled vial tells you nothing you can verify.
- “Research-grade” is not a standard. It is not a grade anyone checks. It does not promise that the powder is GHK-Cu, that it is pure, or that the amount matches the label.
- No calculation fixes this. The math here is exact for the numbers you type. It cannot tell you whether the powder in your vial matches what is printed on it.
What is GHK-Cu?
GHK-Cu is a small lab-made peptide (made of three building blocks, so it is called a tripeptide) that holds onto copper. It is studied in skin, hair, and tissue research.
It is one of the most common cosmetic research peptides and is available in higher vial strengths than most peptides.
Cosmetic and skin research peptides reconstitute the same way as any other peptide. Some carry a natural color once mixed, which is normal and not a sign of a problem.
What published research looked at
No large human clinical trials; the primary evidence is animal (rat) and cell/tissue studies. Not FDA-approved as a drug.
Looked at whether it boosts connective-tissue buildup in healing wounds.
- Tested on
- Rats (implanted wound-chamber model)
- Amount
- Various concentrations (concentration-dependent effect)
- How often
- Sequential injections into the wound chamber
- How long
- Wound-chamber implantation period
- Route
- Local injection
These are study details, not instructions. An amount given to animals cannot be turned into a safe amount for a person.
How much bac water for GHK-Cu?
The chart below is a GHK-Cu reconstitution chart: each common vial size, the bac water to add, and where a 2 mgmeasurement lands on a 1 mL insulin syringe. Use the calculator above for your exact vial and the amount you want to measure.
| Vial amount | Bac water to add | Concentration | Amount to measure | Syringe units |
|---|---|---|---|---|
| 50 mg | 2.5 mL | 20 mg/mL | 2 mg | 10 units |
| 100 mg | 3 mL | 33.33 mg/mL | 2 mg | 6 units |
| 200 mg | 3 mL | 66.67 mg/mL | 2 mg | 3 units |
The amount in the “Amount to measure” column is an example chosen so the math is easy to follow. It is not a recommended amount, and this site does not recommend how much to use. Units assume a U-100 insulin syringe (100 units = 1 mL). Always confirm the amount printed on your own vial.
How to reconstitute GHK-Cu
- 1Gather your supplies
Wash your hands and lay out your GHK-Cu vial, a vial of bacteriostatic water, an insulin syringe, and alcohol prep pads on a clean surface.
- 2Swab both vial tops
Wipe the rubber stopper of both the peptide vial and the bacteriostatic water vial with separate alcohol prep pads. Let them air dry.
- 3Draw the bacteriostatic water
For a 50 mg vial, draw about 2.5 mL of bacteriostatic water. Use the calculator on this page to match your exact vial strength.
- 4Add water to the peptide vial
Insert the needle at an angle and let the water run slowly down the inside wall of the vial. Do not spray it directly onto the powder.
- 5Swirl gently
Roll or swirl the vial between your palms until the powder fully dissolves. Roll it, do not shake it, if your product's instructions say so. The solution should look clear.
- 6Label and refrigerate
Label the vial with the peptide name, the date mixed, and the expiration date, then refrigerate it immediately.
Storage and shelf life
Keep in the fridge. Blue tint is normal. A blue tint after reconstitution is completely normal and comes from the copper. It is not a sign of contamination. Once you add bac water, the peptide slowly breaks down, so refrigerate the vial and discard it if the solution turns cloudy or develops particles.
GHK-Cu reconstitution FAQ
Keep reading about GHK-Cu
- SafetyWhat Happens if You Add Too Much BAC Water
Short Answer Adding too much BAC water does not ruin the peptide. It simply dilutes the concentration, meaning you will need to draw a larger volume per dose. W
- SafetyHow to store reconstituted peptides
Storage Basics Refrigerate all reconstituted peptides at 36 to 46 °F (2 to 8 °C). Store vials upright in the door shelf or a dedicated container, away from the
- GuideWhat you cannot know about your vial
The math is exact for the numbers you type, but it cannot tell you what is really in the powder. What no calculation can verify.
- SafetyBAC water and peptide shelf life
How long reconstituted peptides and opened bac water last, why refrigeration and clean technique both matter, and when to discard a vial.
- Peptide GuideBPC-157 bac water calculator & guide
BPC-157 is a synthetic peptide derived from a protein found in gastric juice, studied in research settings for tissue repair and recovery.
Related peptides
Compare GHK-Cu with Melanotan II side by sideReferences
Primary sources for the facts on this page. We cite regulatory and peer-reviewed authorities rather than secondary blogs.
- Bacteriostatic Water for Injection, USP - prescribing information · U.S. FDA labeling via DailyMed (NIH / NLM)Defines bacteriostatic water as sterile water with 0.9% benzyl alcohol added as a bacteriostatic preservative, supplied in a multiple-dose container for diluting or dissolving drugs; contraindicated in neonates.
- Benzyl alcohol (Compound CID 244) · NIH PubChem, National Library of MedicineChemical identity, properties, and safety data for benzyl alcohol, the bacteriostatic preservative in BAC water.
The Plan Builder turns these numbers into a printable, step-by-step plan. You can also read how reconstitution works, brush up on the bac water calculator, or start from the complete bac water guide.